FP2S51 - Biochemistry and Biotechnology 01 Sep 2020 - 31 Aug 2023 | Version 5

Associated Module Information

Module Code: FP2S51
Module Title: Biochemistry and Biotechnology
Faculty: Faculty of Computing, Engineering and Science
Faculty Group: Biological and Forensic Sciences
Faculty Sub Group: Biological Science
Module Leader: Paul Jones
Module Team: Jeremy Lewis, Samantha O'Connell, Deborah Crockard, Hannah Minton, Helen Burn
First Intended Intake: AUG 2016 Final Year of Intake:
Date Closed:
Credit Value: 20 Credit Level: 5
Language: English
Percentage of Module Taught in Welsh: 0
Equivalent Module:
HECOS codes: 100388 - forensic science
HECOS Code Weighting: 100

Document Version Information

Version 5
Valid From 01 Sep 2020
Valid To 31 Aug 2023

Module Aims

1. To introduce students to DNA profiling techniques and the National DNA database, and selected other topics in the fields of biochemistry and biotechnology.

2. To give the student practical laboratory experience in biochemical and microbiological techniques.

3. To give students the opportunity for group work during a research exercise into a biotechnology company.

Content Summary

DNA Profiling and the National DNA database: Historical development of DNA profiling and its use in human identification and forensic investigation. DNA profiling using multilocus and single locus probes. PCR, STR profiling, mitochondrial DNA analysis, low copy number DNA analysis. The role of the NDNAD, legislation and use as an investigative tool. Limitations of the NDNAD.

Immune system: Innate and adaptive immunity Cells of the immune system Lymphoid tissues and organs Soluble factors: antibodies, cytokines etc Key systems: complement, the MHC complex Measurement of effectors of the immune response Immune interactions Antigen recognition and lymphocyte activation Complement activation and consequences Inflammation. Antibody-mediated effector mechanisms. Cell-mediated effector mechanisms. Central role of B and T cells. Immunity and infectious diseases eg AIDS. Autoimmune responses. Overview of immunoassays for qualitative and quantitiative analysis of proteins eg identifying suspect meat in processed meat products.

Metabolism: Basic concepts and design. Overview of coupled, interconnecting reactions. The oxidation of carbon fuels as a source of cellular energy. Metabolic pathways and the recurring motifs. Metabolism of poisons and toxins and identification of metabolites as markers.

Enzymes: basic concepts and Kinetics. Specificity of catalysts. The Michaelis-Menton model account for the kinetic properties of enzymes. Inhibition of enzymes by specific molecules and substances. Vitamins as precursors to coenzymes.

Biotechnology and gene manipulation: Overview of ethical and social issues surrounding gene technology. Plasmids and their structure. Plasmid DNA as vectors. Restriction enzymes Producing recombinant DNA. Sticky and blunt ends.

Learning and Teaching Methods

Activity Type Hours
Lecture 24
Tutorial 12
Practical classes and workshops 10
Independent Study 108
Directed Study 46
Total Hours Selected 200

Learning Outcomes

# Learning Outcome
LO1 Understand the cellular and molecular foundation of living things and have an appreciation of the fundamentals of genetics.
LO2 Appreciate some of the ways in which biochemistry and biotechnology can be applied in practice to forensic or analytical science.

Module Requisites

N/A

Assessment Criteria

Assessment Category Assessment Type Description Duration Word Count Weight (%) Best of? Pass Mark
Written Examination Written Examination - Open Book (Unseen) 1 Open book with an extra 30 minutes provided to students for technical issues with uploading via Turnitin. 50 N/A 40 No 40
Practical Assessment (EX) Practical Examination 1 Practical Work 480 N/A 60 No 40

Assessment Matrix

Assessment Type Learning Outcomes
LO1 LO2
Written Examination - Open Book (Unseen) 1
Practical Examination 1

Reading List

Klug and Cummings. Essentials of genetics.

Raven, Johnson, Singer & Losos. Biology.

Lincoln, Forensic DNA Profiling Protocols (Methods in Molecular Biology)

Primrose, Twyman and Old. Principles of Gene Manipulation.

Molecular Biotechnology: Principles and Applications of Recombinant DNA.

B.J.Glick and J.J Pasternack. Molecular Biotechnology: Principles and Applications of Recombinant DNA.