PH3S113 - Advanced Analytical Techniques 01 Jul 2022 - 31 Aug 2028 | Version 1
Associated Module Information
| Module Code: | PH3S113 | ||
|---|---|---|---|
| Module Title: | Advanced Analytical Techniques | ||
| Faculty: | Faculty of Computing, Engineering and Science | ||
| Faculty Group: | Biological and Forensic Sciences | ||
| Faculty Sub Group: | Biological Science | ||
| Module Leader: | Andrew Graham | ||
| Module Team: | Suzanna Kean, Rehana Karim, Peter Miedziak, Meirion Pugh, Antony Davies | ||
| First Intended Intake: | SEP 2022 | Final Year of Intake: | 2027 |
| Date Closed: | |||
| Credit Value: | 20 | Credit Level: | 6 |
| Language: | English | ||
| Percentage of Module Taught in Welsh: | 0 | ||
| Equivalent Module: | |||
| HECOS codes: | 100413 - analytical chemistry | ||
| HECOS Code Weighting: | 100 | ||
Document Version Information
| Version | 1 |
|---|---|
| Valid From | 01 Jul 2022 |
| Valid To | 31 Aug 2028 |
Module Aims
To broaden the student’s knowledge of analytical chemistry and practical skills through the study of modern applied methods of analysis.
To develop the student’s ability to critically assess the relative merits of named analytical techniques and analytical data
Content Summary
1) Advanced Separation Theory
Building upon theory from previous years: The importance of resolution and definition of peak shape in quantitative analysis by GC and HPLC. Column performance in relation to the van Deemter equation and related chromatographic parameters and their extension to the optimisation of SPE procedures. Advanced SPE procedures for complex matrices and modern developments in sample preparation.
Normalisation of retention times in relation to column performance. The use of Kovats retention indices and automated database searching in modern analyte identification
2) Advanced Separation Methods
Liquid chromatography-Mass Spectrometry (LC-MS)
LC – a review of the functions of key modules in a HPLC system, the use of different mobile phases, together with the benefits of using mobile phase additives, gradient vs isocratic elution, and, the role of column chemistry when selecting a column and differences between different column types.
Interface – theory of how LC eluent can be introduced into a MS, a consideration of different ionisation interfaces - ESI and APCI.
MS – a review of MS detection, introduction to tandem mass spectrometry – LC-QQQ and LC-TOF/LC-QTOF, an appreciation of the increasing use of high-resolution, accurate-mass (HR/AM) data.
Acquisition modes – the benefits of using SIM or (D)MRM.
Applications of LC-MS in chemical analysis, pharmaceutical science and forensic toxicology.
The process of method development will be discussed, including the steps of method validation;-
• assessment of recovery and ion suppression,
• assessment of precision, accuracy, stability and carryover,
• linearity at higher levels,
• stability of samples and intermediary extracts in storage when relevant
,Statistical analysis
• proficiency Testing samples.
These topics will be discussed in relation to current regulatory standards, e.g. ISO17025.
3) Advanced Spectroscopic Techniques
NMR as an analytical tool: q-NMR and NMR for enantiomeric resolution: choice of standards (q-NMR), agents for enantiomeric resolution, sample preparation, experimental considerations, data interpretation, utility in comparison with other analytical techniques, industrial applications.
Emission and Fluorescence Spectroscopy:- Introduction to emission spectroscopy including flame and plasma emission spectroscopy and a comparison with atomic absorption spectroscopy. Applications of emission spectroscopy in elemental analysis and chemical speciation (spectral line sensitivity vs detection requirements vs interfering ions). - Basic principles of fluorescence spectroscopy
Introduction to Ion Mobility Spectrometry;- Infrared and Raman Imaging and Terahertz Spectroscopy with applications to pharmaceutical, environmental and forensic sciences as well as manufacturing.
Learning and Teaching Methods
| Activity Type | Hours |
|---|---|
| Lecture | 24 |
| Practical classes and workshops | 10 |
| Independent Study | 50 |
| Directed Study | 102 |
| Problem / challenge based learning | 14 |
| Total Hours Selected | 200 |
Learning Outcomes
| # | Learning Outcome |
|---|---|
| LO1 | To critically assess and develop methods to solve problems in chemical analysis |
| LO2 | To select techniques and interpret data to provide accurate and effective analysis of a given chemical sample |
Module Requisites
| Code | Title | Requisite Type |
|---|---|---|
| MOD012492 | Modern Techniques in Analysis and Identification | pre-requisite |
Assessment Criteria
| Assessment Category | Assessment Type | Description | Duration | Word Count | Weight (%) | Best of? | Pass Mark |
|---|---|---|---|---|---|---|---|
| Synchronous Onsite Practical Assessment | Practical Coursework (Onsite) 1 | A guided assessment demonstrating the application of practical skills and problem solving gained over a series of practical | 0 | 2000 | 50 | No | 40 |
| Asynchronous Assessment | Report 1 | Written assignment allowing demonstration of the taught theory of the module | 0 | 2000 | 50 | No | 40 |
Assessment Matrix
| Assessment Type | Learning Outcomes | ||
|---|---|---|---|
| LO1 | LO2 | ||
| Practical Coursework (Onsite) 1 | ✔ | ✔ | |
| Report 1 | ✔ | ✔ | |